太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究     

曲武  洪国粦  赵晶 《海洋学报(英文版)》2018,37(6):104-111

本文在太平洋深海沉积物中分离得到一株孔雀石绿降解菌株，鉴定命名为Tenacibaculum sp.HMG1。通过菌株生长实验和高效液相色谱的研究表明， HMG1菌株可以在20 mg/L的孔雀石绿中维持较快的生长速率，并且在12 h内可降解98.8%的孔雀石绿，这证明该菌株具有很高的孔雀石绿耐受能力和降解活性。通过基因组测序在HMG1菌株发现一条过氧化物酶基因可能参与了孔雀石绿的降解，随后利用原核表达获得了相应的重组蛋白。实验表明，该重组过氧化物酶具有极强的活性，可在1000 mg/L的孔雀石绿中发挥降解功能。本文利用液相色谱-质谱联用（LC-MS）技术对孔雀石绿的菌株降解产物和重组酶降解产物进行鉴定，并基于鉴定结果推测了两种降解途径。结果发现两种降解方式存在共同的降解途径。此外，孔雀石绿降解条件的实验结果证明重组过氧化物酶可以在低温（20℃）、复杂的pH值（6.0–9.0）、高盐度（100 mmol/L）、金属离子和EDTA等反应条件下依旧维持很高的孔雀石绿降解活性。以上实验结果表明，HMG1菌株和重组过氧化物酶均在孔雀石绿污染生物修复方面具有很大潜力。  相似文献   

Changes of Biogenic Elements in Phragmites australis and Suaeda salsa from Salt Marshes in Yellow River Delta,China     

JIA Jia  BAI Junhong  WANG Wei  ZHANG Guangliang  WANG Xin  ZHAO Qingqing  ZHANG Shuai 《中国地理科学(英文版)》2018,(3)

Little information is available on biogenic elements(carbon, nitrogen, phosphorus and sulfur) and the ecological stoichiometric characteristics of plants in coastal wetlands. To investigate the contents of carbon, nitrogen, phosphorus and sulfur of plants, and their ecological stoichiometric characteristics in the Yellow(Huanghe) River Delta, plant samples were collected from two typical salt marshes(Suaeda salsa and Phragmites australis wetlands) during the period of from August to October in 2007, and the ratios of C/N, C/P, N/P, C/N/P and C/N/P/S were calculated. Results showed that during the studying period, plant C, N and P were lower than the global average values, and plant N and P were lower than the China's average values. Leaf C and S in Suaeda salsa were significantly lower than those in Phragmites australis(P 0.05), and leaf N and P in Suaeda salsa and Phragmites australis showed no significant differences(P 0.05). Average C/N ratios were 23.75 in leaf, 73.36 in stem, 65.67 in root of Suaeda salsa, and 33.77 in leaf, 121.68 in stem, 97.13 in root of Phragmites australis. Average C/N ratios of Suaeda salsa and Phragmites australis were all great than 25, indicating the salt marsh in the Yellow River Delta is an N limitation system. Average C/P ratios were 276.78 in leaf, 709.28 in stem and 1031.32 in root of Suaeda salsa, and 536.94 in leaf, 768.13 in stem and 875.22 in root of Phragmites australis. The average N/P ratios of Suaeda salsa were 12.92 in leaf, 10.77 in stem and 10.91 in root, and the average N/P ratios of Phragmites australis were 16.40 in leaf, 7.40 in stem and 6.92 in root, indicating the Suaeda salsa wetlands were N limited and Phragmites australis wetlands were N limited in August and P limited in October in 2007. The average C/N, C/P and C/N/P ratios in Suaeda salsa and Pragmites australis were higher than the global average values, indicating the lower quality of organic matter provided by wetland plants in the Yellow River delta.  相似文献   

基于GNSS的LEO卫星TRAIM可用性算法及分析     

马洋  李延东  王韶波  蒙薇  赵黎晔 《大地测量与地球动力学》2018,38(8):832-835

详细研究了基于GNSS的LEO卫星接收机授时自主完好性（TRAIM）故障检测数学模型以及可用性算法。以GPS和BDS组合为例，分别对500 km、650 km、800 km和1 400 km等几种典型轨道高度的卫星，开展基于GNSS的LEO卫星TRAIM可用性仿真分析。结果表明，在告警阈值100 ns时，基于GPS+BDS组合系统的TRAIM检测可用概率达到99.1%以上，基本满足LEO卫星TRIAM检测可用性需求。  相似文献   

基于闭合环的InSAR解缠误差探测方法研究     

王霞迎  赵超英  刘媛媛  谷守周 《大地测量与地球动力学》2018,38(3):321-325

基于Sentinel-1数据，提出一种基于多个干涉图闭合环的残差探测解缠误差方法。以形变量较大的墨西哥城区为实验场，分别采用不同的闭合环对同一个干涉对进行探测，结果具有较好的符合性。  相似文献   

内蒙古鲁新井田水文地质条件研究          下载免费PDF全文

赵志强  安美艳  高奎锋  王兆亮  王福芝 《山东国土资源》2018,34(6)

矿产资源开发导致了地下水失衡,地下水失衡又给矿产开发造成了极大的安全隐患,近年来,矿产资源开发与地下水环境保护之间的矛盾愈发凸显。通过对内蒙古鲁新井田典型的水文地质条件进行分析研究,分析采矿导致地下水失衡机理,深入剖析矿井开采充水条件及矿山开发对地下水环境的影响,合理提出了促进矿产开发与地下水保护相互协调的对策建议,为实现"采矿保水"协调统一提供了基础地质依据。为类似地区矿产开发过程中遵循自然规律,趋利避害,保障生产安全,保护地下水环境安全,实现资源绿色开发有较好的指导借鉴作用。  相似文献   

Soil Organic Carbon Contents and Stocks in Coastal Salt Marshes with Spartina alterniflora Following an Invasion Chronosequence in the Yellow River Delta,China     

ZHANG Guangliang  BAI Junhong  JIA Jia  WANG Xin  WANG Wei  ZHAO Qingqing  ZHANG Shuai 《中国地理科学(英文版)》2018,(3)

Plant invasion alters the fundamental structure and function of native ecosystems by affecting the biogeochemical pools and fluxes of materials and energy. Native(Suaeda salsa) and invasive(Spartina alterniflora) salt marshes were selected to study the effects of Spartina alterniflora invasion on soil organic carbon(SOC) contents and stocks in the Yellow River Delta. Results showed that the SOC contents(g/kg) and stocks(kg/m~2) were significantly increased(P 0.05) after Spartina alterniflora invasion of seven years, especially for the surface soil layer(0–20 cm). The SOC contents exhibited an even distribution along the soil profiles in native salt marshes, while the SOC contents were gradually decreased with depth after Spartina alterniflora invasion of seven years. The natural ln response ratios(Ln RR) were applied to identify the effects of short-term Spartina alterniflora invasion on the SOC stocks. We also found that Spartina alterniflora invasion might cause soil organic carbon losses in a short-term phase(2–4 years in this study) due to the negative Ln RR values, especially for 20–60 cm depth. And the SOCD in surface layer(0–20 cm) do not increase linearly with the invasive age. Spearman correlation analysis revealed that silt + clay content was exponentially related with SOC in surface layer(Adjusted R~2 = 0.43, P 0.001), suggesting that soil texture could play a key role in SOC sequestration of coastal salt marshes.  相似文献   

海洋卫星军民融合发展现状及几点思考          下载免费PDF全文

李科  张靓  魏兰  赵忠东 《海洋测绘》2018,(3):78-82

对比分析了国内外海洋卫星军民融合发展现状,指出了我国存在的主要差距,并分析造成这些差距和障碍的主要问题和症结所在,从标准体系融合、技术融合和数据融合三个方面提出我国海洋卫星军民融合发展思路,并结合发展思路提出了科学统筹、科技引领、开放协作、以人为本的军民融合发展举措,为海洋卫星军民融合发展提供了借鉴作用和决策支持。  相似文献   

热带西太平洋砂壳纤毛虫的多样性     

李海波  张武昌  赵苑  赵丽  董逸  王超锋  梁晨  肖天 《海洋学报(英文版)》2018,37(10):218-228

为了探明热带西太平洋海区砂壳纤毛虫的多样性、垂直分布及纬向变化，我们于2012年11-12月在该海区四个断面采集了0-200 m的水样。共检出砂壳纤毛虫39属124种，多数种类喜好生活在表层和次表层。砂壳纤毛虫种丰富度、丰度以及多样性指数的高值主要出现在比叶绿素浓度最大值稍浅的位置，与温度和叶绿素浓度显著正相关，与盐度和采样深度显著负相关。多数优势种与环境因子间的相关性不显著。热带西太平洋海区砂壳纤毛虫多样性极高，各站种丰富度在25-52之间。在从表层至75 m处的多数采样点香农指数均大于3。该海区砂壳纤毛虫冗余种的比例很高，占砂壳纤毛虫总种类数的87.90%，贡献了砂壳纤毛虫60.38%的丰度，表明热带西太平洋海区砂壳纤毛虫群落对饵料组成和摄食压力改变的应对能力很强，稳定性很高。  相似文献   

多种海洋开发类型的综合效益评估     

高升  徐敏  刘佰琼  曹广喜  赵林 《海洋通报》2018,(1):17-24

随着海洋开发活动的不断加剧,各海洋开发类型综合效益的评估与分析对维持海洋社会经济与海洋生态环境的可持续发展具有重大意义,不同海洋开发活动带来不同的社会、经济效益,也产生了海洋资源的损耗,影响了海洋环境和海洋生态服务功能。根据江苏省海洋资源开发活动特点,筛选出江苏海岸带资源开发中常见的4种典型的开发利用类型,选取了能够突出反映海洋开发活动综合效益的经济效益指标、社会效益指标、资源损耗指标、环境成本指标构建海洋开发类型综合效益评估体系。基于层次分析法与熵权法组合权重,运用综合效益指数评价模型分别计算了单一指标效益值、准则层效益值、各研究案例效益值、各用海类型效益值并对以上研究数据之间的相互关系进行了深入分析,探求各用海类型综合效益评估值的关键影响因素。通过实证研究发现4种海洋开发类型的综合效益评估值均介于0.4~0.6之间,处于一般的综合效益状态,综合效益从高到底依次排序:临港工业用海类型为0.551 3、海上风电用海类型为0.492 4、排污倾倒用海类型为0.441、围海养殖用海类型为0.434 3。海洋开发类型综合效益的评估对及时调整海洋开发对策,缓和海洋开发与海洋生态环境的矛盾具有积极意义。  相似文献   

Total Synthesis of Marine Cyclic Enol-Phosphotriester Salinipostin Compounds     

ZHAO Mingliang  WEI Xianfeng  LIU Xuemeng  DONG Xueyang  YU Rilei  WAN Shengbiao  JIANG Tao 《中国海洋大学学报(英文版)》2018,(3)

Due to their structural diversity and variety of biological activities, marine natural products have been the subject of extensive study. These compounds, especially phospholipid polycyclic aromatic hydrocarbons, have a wide range of pharmacological applications, including embedded DNA and central nervous system, anti-tumor, anti-virus, anti-parasite, anti-bacterial, and antithrombotic effects. Unfortunately, the insufficient drug sources have limited the development of these compounds. In this study, we isolated salinpostin compounds from a fermentation solution of marine-derived Salinospora sp., which has a common bicyclic enol-phosphotriester core framework, as well as potent and selective antimalarial activities against P. falciparum with EC_(50)=50 nmolL~(-1). The chemical synthesis of these compounds in greater quantities is necessary for their use in bioactivity studies. Thus we explored a short route with high yields and mild reaction conditions, which can generate combinatorial libraries for drug discovery and lead optimization. We developed a new total synthesis method for six cyclic enol-phosphotriester salinipotin compounds and their diastereomers. For the total synthesis of cyclipostin P, we prepared cyclic enol-phosphotriester salinipostin compounds in 10 steps from a readily accessible starting material, 1,3-dihydroxyacetone, and obtained an overall yield of 1.29%. We fully characterized these compounds by proton nuclear magnetic resonance(~1H-NMR), carbon-13 NMR(~(13)C-NMR), and high-resolution mass spectrometry(HRMS) analyses, and found they coincide absolutely with the same compounds reported previously.  相似文献